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Plasmid-Safe? ATP-Dependent DNase/ATP依賴的DNA酶
| 貨號(hào) | E3101K/E3110K | 售價(jià)（元） | 1230/6462 |
| 規(guī)格 | 1000U/10000U | CAS號(hào) |
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EpiScript? RNase H- Reverse Transcriptase/EpiScript? RNase H-逆轉(zhuǎn)錄酶 |
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Plasmid-Safe? ATP-Dependent DNase/ATP依賴的DNA酶 |
1000U/10000U |
1230/6462 |
Plasmid-Safe? ATP-Dependent DNase selectively hydrolyzes linear double-stranded (ds) DNA to deoxynucleotides at slightly alkaline pH and, with a lower efficiency, linear and closed-circular single-stranded DNAs. The reaction is ATP-dependent, and does not affect closed-circular supercoiled or nicked circular dsDNAs. The enzyme can be conveniently and completely heat-inactivated by a 30 minute incubation at 70°C. Plasmid-Safe DNase is useful as a final “cleanup” of DNA preparations from plasmid and cosmid clones, to avoid the problems caused by contaminating genomic DNA.
粘粒、BAC、fosmid和質(zhì)粒制備物中去除污染的細(xì)菌染色體DNA。該酶將從末端逐步降解線性DNA；閉環(huán)DNA(例如質(zhì)粒)不具有游離末端，因此不會(huì)被降解。這些特性使得Plasmid-Safe ATP-Dependent DNase成為BAC和fosmid純化方案的理想選擇，例如用于鳥(niǎo)槍測(cè)序，以及其他需要高純度DNA的應(yīng)用。
優(yōu)點(diǎn)：
- Digest contaminating linear DNA without digesting nicked or closed-circular dsDNA or supercoiled DNA (plasmids, fosmids, etc.)；
- Use as a final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations
- Utilize whenever protecting circular dsDNA is important
- 消化污染的線性DNA，而不消化有缺口或封閉的圓形dsDNA或超螺旋DNA（質(zhì)粒，粘粒等）；
- 用作質(zhì)粒，cosmid, fosmid，BAC載體和克隆制劑的最終純化步驟；
- 在保護(hù)重要環(huán)狀dsDNA時(shí)使用.
組成成分：
? Plasmid-Safe? ATPDependent DNase （10U/μL）：1mL； -20°C
? Plasmid-Safe? 10X Buffer：5mL；-20°C
? ATP Solution (25 mM)：2m L； -20°C